![]() ![]() Moso bamboo ( Phyllostachys edulis) is one of the most important non-timber rhizomatous forestry plants in the world, sustaining the livelihood of approximately 2.5 billion people (Peng et al., 2013). Taken together, these results suggest that post-transcriptional regulation may potentially have a vital role in the underground rhizome-root system. Several hundred differential poly(A) sites in the rhizome-root system were identified. Furthermore, this study provided a quantitative atlas of poly(A) usage. Further analysis of intronic polyadenylation revealed that LTR/Gypsy and LTR/Copia were two major transposable elements within the intronic polyadenylation region. ![]() In addition, we characterized 25 069 polyadenylation sites from 11 450 genes, 6311 of which have APA sites. Notably, more than 42 280 distinct splicing isoforms were derived from 128 667 intron-containing full-length FLNC reads, including a large number of AS events associated with rhizome systems. In total, 145 522 mapped full-length non-chimeric (FLNC) reads were analyzed, resulting in the correction of 2241 mis-annotated genes and the identification of 8091 previously unannotated loci. We therefore used a combination of single-molecule long-read sequencing technology and polyadenylation site sequencing (PAS-seq) to re-annotate the bamboo genome, and identify genome-wide alternative splicing (AS) and alternative polyadenylation (APA) in the rhizome system. However, the post-transcriptional mechanism for the development of the rhizome system in bamboo has not been comprehensively studied. Moso bamboo ( Phyllostachys edulis) represents one of the fastest-spreading plants in the world, due in part to its well-developed rhizome system. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |